- Available Technologies
Extra-chromosomal circular DNA (eccDNA) replicons deliver and express foreign DNA, which may include gene therapy targets and DNA editing reagents, outside of the main chromatin body. EccDNA are a vehicle for ultra-high gene expression. Gene therapy technologies such as CRISPR/Cas9 are projected to grow beyond $50 billion by 2023. Similarly, the agrobiotechnological industry spends approximately $40 million registering each biotechnological trait used in crops. With nearly 500 agrobiotechnological traits registered globally, eccDNA may provide an attractive means to circumvent these regulations. It may also provide a route to develop improvements in current human gene therapy technologies as eccDNA technology is developed in mammalian systems.
Gene therapy, agrobiotechnology
The eccDNA plant replicon can be utilized as a vehicle for transferring and enhancing beneficial traits in crops. The extra nuclear replicon is a highly dynamic unit that evolved naturally in Palmer amaranth as an adaptive survival mechanism to rapidly increase gene copy number of the EPSPS gene in response to the herbicide glyphosate. Over expression of this gene Palmer amaranth, confers resistance to glyphosate. The invention allows for the over expression of a target genes and biochemical pathways as desired, especially where gene dosage enhances a desired trait. EPSPS may be maintained in the replicon to use glyphosate as a selectable marker for plant biotechnology. This invention solves, the problem of transgene recalcitrance. Knowledge of this plant replicon can be used to tailor species-specific replicons, and also opens the door to transgene stacking and exploiting precision trait enhancement, with effects that may be analogous to heterosis. Such a system could be described as analogous and complimentary to the Crispr-CAS9 gene editing system.
Dr. Christopher A Saski, William T Molin
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