Multi-Needle Roller for Cell Seeding

Technical Summary:

The device described herein comprises a handle to which a cylindrical fluid holder is attached, and a rotating drum which slides over the fluid holder. The rotating drum has multiple hollow needle tips attached to its surface. The lumen of the hollow needle tips communicate with the internal surface of the rotating drum. The cylindrical fluid holder is adapted with a fitting allowing for cell suspensions to be infused into the fluid holder using a syringe. The fluid holder has one or more rows of holes which align with the lumen of the needles when the device is rolled, allowing fluids to flow from the cylindrical fluid holder through the lumen of the hollow needles and be injected into the tissue on which it is being rolled. The action of rolling the device while applying pressure over a surface such as a living tissue or a scaffold pierces the substrate and delivers the cells via the multiple needle tips.