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Animal health diagnostics, mammary development models
This method uses a sterile, fine-needle biopsy device to collect mammary tissue samples once a week during the course of early development. The samples are either paraffin embedded and stained for use in fluorescence microscopy or RNA isolation is performed to evaluate changes in cell signaling over time. This data is then used as a genomic indicator of animal productivity. Recently sequenced transcriptomic data has shown significantly up and down-regulated genes beginning in one-week old animals. The predictive genetic value provided from gene expression will vastly increase the degree of sensitivity at the molecular level of evaluation. Lastly, a comprehensive prediction can be made regarding the animal’s health and productivity during its life post-development. This method is repeatable and reproducible, low-cost, and has the potential to allow early prediction of animal health unlike traditional methods of post-mortem analysis.
Animal study validation
Heather Dunn, Kathryn A Elliott, Thomas Scott, Matthew Burns
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