- Available Technology
Real-time, live cell imaging
In this method of super-resolution microscopy, interleaved solid and doughnut-shaped laser pulses are used to excite the biological sample, and the signal generated from each pulse is recorded. The signals between neighboring pulses are subtracted to effectively yield a shrunken point spread function, providing improved spatial resolution. The improvement factor is over two without deconvolution. This method uses only one laser at a single wavelength, which removes the need for high powered lasers used by other super-resolution microscopy techniques. The reduced power allows for imaging of live samples without damaging them. Low power pulsed lasers on the market cover a wide range of wavelengths, spanning from UV to near infrared. This allows for an unlimited selection of dyes or chromophores to use during the imaging process
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