{xtd_wp_head}
Skip to content
About
Technology
Innovators
Students
Industry
Startups

Market Overview

Fluorescence recovery after photobleaching (FRAP) is a versatile and widely used technique for measuring molecular diffusion properties within solutions, cells, tissues, biomaterials and the food and pharmaceutical industries. While it is a standard tool in biomedical and environmental research, all existing FRAP systems can only measure 2D diffusion, providing only diffusion measurement in limited directions. The long-standing goal to create a 3D diffusion tensor has not yet been achieved. The global Light Sheet Microscopy market segment was valued at $101.2 million in 2020 at a CAGR of 10.3% and is projected to grow to $181.9 million in 2026. This can be associated with application for a 3D FRAP microscope in the Biomaterials market segment, which was valued at $105.18 billion in 2019 at a CAGR of 14.5%. Clemson University researchers have implemented the first 3D FRAP microscope in the world by innovatively combining the 3D spatial Fourier transform FRAP method with high speed volumetric imaging and high-resolution structural imaging, calling it Light-sheet imaging-based Fourier transform FRAP (LiFT-FRAP).
Dr. Hai Yao
Dr. Tong Ye
2020-022

Applications:

FRAP, 3D Diffusion Tensor, Light-sheet Microscopy

Technical Summary:

This technology incorporates high-speed 3D two-photon scanned light-sheet microscopy with the 3D Fourier transform FRAP method and achieves noninvasive, in-situ measurement of 3D diffusion tensors with diffusivities up to 51 µm2/s. This sensitivity is within the diffusion range of most molecules in biological systems, making FRAP a practical tool for 3D diffusion analysis in broad biomedical applications. A customized control software is written to run the microscope for performing the 3D FRAP experiment; included data analysis package provides a seamless process of acquired images for calculation of 3D diffusion tensors.

Advantages:

• LiFT-FRAP allows for quantification in any direction in a 3D space
• The microscope provides for a much higher volumetric imaging speed than the current available approach for FRAP, therefore LiFT-FRAP can measure a wide range of molecular diffusivities
• This approach avoids the previously necessary tissue sectioning, plus all of the structural integrity problems and material property changes

Download Printable PDF

Technology Overview

State of Development

Prototype

Category

Biotechnology

Serial Number

63/052,726

CURF Reference No.

2020-022

Inventors

Dr. Hai Yao, Dr. Tong Ye


For More Info, Contact:

Interested in this technology?
Contact curf@clemson.edu
Please put technology ID in subject line of email.

Contact

Get Started
Ready to Get Started?

Contact our team at CURF

Contact CURF