- Available Technology
FRAP, 3D Diffusion Tensor, Light-sheet Microscopy
This technology incorporates high-speed 3D two-photon scanned light-sheet microscopy with the 3D Fourier transform FRAP method and achieves noninvasive, in-situ measurement of 3D diffusion tensors with diffusivities up to 51 µm2/s. This sensitivity is within the diffusion range of most molecules in biological systems, making FRAP a practical tool for 3D diffusion analysis in broad biomedical applications. A customized control software is written to run the microscope for performing the 3D FRAP experiment; included data analysis package provides a seamless process of acquired images for calculation of 3D diffusion tensors.
• LiFT-FRAP allows for quantification in any direction in a 3D space
• The microscope provides for a much higher volumetric imaging speed than the current available approach for FRAP, therefore LiFT-FRAP can measure a wide range of molecular diffusivities
• This approach avoids the previously necessary tissue sectioning, plus all of the structural integrity problems and material property changes
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